Gene synthesis training

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A practical introduction to gene synthesis

Gene synthesis technologies are rapidly changing research paradigms in molecular biology. The purpose of this gene synthesis training is to provide students a practical understanding of gene synthesis so that they can make the most of it and better interact with providers of gene synthesis services.

Background

The development of recombinant DNA technology revolutionized biology, allowing researchers to construct expression vectors and introduce them into cells to express foreign genes or native genes controlled by new promoters. Until recently, construction of expression vectors was routinely accomplished by ligating together DNA fragments generated by restriction enzyme digestion. However, traditional recombinant DNA methods are mostly limited to recombining natural DNA fragments to produce the final product.

Two developments have made it possible for any molecular biology laboratory to synthesize and clone genes without the need for a DNA template. First, is the ability to perform template-free polymerase cycling/chain assembly (PCA). PCA is accomplished using a mix of oligonucleotides (oligos) that overlap and anneal to form a long DNA chain. The second development important for gene synthesis is ligation-independent cloning (LIC) methods. LIC allows one to clone DNA fragments without restriction digests or, in some cases, sequence constraints.

Template- and restriction enzyme-free gene synthesis removes the restrictions of traditional cloning methods and allows researchers to generate completely new DNA sequences limited only by the design rather than the methods.

Objective of the gene synthesis training

The purpose of this gene synthesis training is to provide the students with a thorough understanding of the different techniques involved in the completion of a gene synthesis project. This will be achieved through a combination of lectures and laboratory work leading to the complete synthesis of a large DNA fragement. Students are expected to have basic knowledge in molecular biology or biochemistry, but the course is designed to accommodate students who have no practical molecular biology experience.

At the end of the course, students will be able to better understand the potential and limitations of current gene synthesis technologies,

Target Audience

  •  This continuous education course has been developed for biotechnology professionals who want to better understand recent developments in gene synthesis.
  • Graduate students, post-graduate fellows, and faculty with expertise in engineering or quantitative sciences interested moving into synthetic biology may find this course useful to give them a first exposure to molecular biology techniques they would need to support their research.

Price and Registration

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