Noise in synthetic genetic oscillators

This paper focuses on characterizing the stochastic dynamics of previously described synthetic genetic oscillators. It is a very nice combination of experimental and computational work. The computational aspect includes both image analysis and parameter estimation. They breakdown the noise into different components including intrinsic noise, cell cycle noise, and parameter variability by looking at the expression […]

CRISPR-Cas9:Rational design of highly active sgRNAs

A group led by David Root from the Broad Institute published a nice paper published in Nature Biotechnology (10.1038/nbt.3026) describing a predictive model of the sgRNA target sequence cleavage efficiency.  They created a pool of sgRNAs, tiling across all possible target sites of a panel of six endogenous mouse and three endogenous human genes and quantitatively […]