Monthly Archives: August 2017

//August

gene deletion & gene fusion protocol for yeast

Peccoud Lab Protocols: using PCR to delete a yeast gene or fuse a tag to a gene Introduction Yeast gene deletions or gene fusions (e.g. ORF with epitope tags or FP, promoter replacement, MS2 mRNA tagging) can be generated simply by transformation into yeast of a PCR amplicon containing the desired selectable marker or tag flanked [...]

gene deletion & gene fusion protocol for yeast2019-02-06T19:37:38+00:00

C-terminal tag protocol: Adding a C-terminal tag to a gene

Peccoud Lab Protocol: designing primers to add a C-terminal tag to the (3') end of a gene via PCR Introduction It is sometimes necessary to generate gene fusion strains with a C-terminal tag or promoter changes by intoducing a PCR amplicon of a selectable marker flanked by DNA sequences homologous to the the last 40-60 bp of [...]

C-terminal tag protocol: Adding a C-terminal tag to a gene2019-02-06T19:37:38+00:00

N-terminal tag protocol: Adding an N-terminal tag to a gene

Peccoud Lab Protocol: designing primers to add an N-terminal tag to the 5' end of a gene via PCR Introduction It is sometimes necessary to generate gene fusion strains with an N-terminal tag or promoter changes by introducing a PCR amplicon of a selectable marker flanked by DNA sequences of 40-60 bp homologous to the 5' [...]

N-terminal tag protocol: Adding an N-terminal tag to a gene2019-02-06T19:37:38+00:00

New synthetic biology journal

Why a new synthetic biology journal? Most synthetic biology papers are not published in a synthetic biology journal. They are still published in non-specialized journals that span a broad spectrum of scientific specialties from bioinformatics to molecular biology and biotechnology. It can be difficult for readers to notice synthetic biology papers among all the other papers [...]

New synthetic biology journal2019-02-06T19:37:38+00:00