Biology is becoming increasingly digitized. We rely on computers to operate laboratory equipment, design genetic constructs, and store, share, and analyze genetic data. Cyberbiosecurity is a new field of science that explores novel risks at the interface of biology and computer science. The Peccoud lab is investigating cyberbiosecurity in a variety ways. These include: Applying [...]
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So far Jenna Gallegos has created 15 blog entries.
Last week, I was invited to speak on a panel about synthetic biology and sustainability at the External Advisory Board Meeting of the NSF-funded Socio-Environmental Synthesis Center (SESYNC) at The University of Maryland. SESYNC brings together the science of the natural world with the science of human behavior and decision making to find solutions to complex environmental [...]
A new project in the Peccoud Lab focuses on applying digital signatures to DNA in living organisms. Last week, postdoc Jenna Gallegos gave a presentation at the Synthetic Biology, Evolution, Engineering, and Design meeting in ScottsDale, Arizona describing this work. Abstract DNA molecules are frequently shared within the life sciences community, sometimes changing hands many times with [...]
We've just published a new paper describing the security risks that come along with the increasing digitization of biotechnology workflows. We call this emerging field cyberbiosecurity. Our paper about cyberbiosecurity was published in the Cell Press journal Trends in Biotechnology on December 7th. Concurrent with the publication, we also issued a press release and wrote [...]
Peccoud Lab Retreat 2018 was a big success This weekend, the Peccoud lab went on a three-day retreat at the Cliff House Hotel in Manitou, CO. A retreat for a small academic lab may sound like an unusual idea, but business retreats are common, and our lab strives to operate like a small business. On [...]
Peccoud Lab Protocols: using PCR to delete a yeast gene or fuse a tag to a gene Introduction Yeast gene deletions or gene fusions (e.g. ORF with epitope tags or FP, promoter replacement, MS2 mRNA tagging) can be generated simply by transformation into yeast of a PCR amplicon containing the desired selectable marker or tag flanked [...]
Peccoud Lab Protocol: designing primers to add a C-terminal tag to the (3') end of a gene via PCR Introduction It is sometimes necessary to generate gene fusion strains with a C-terminal tag or promoter changes by intoducing a PCR amplicon of a selectable marker flanked by DNA sequences homologous to the the last 40-60 bp of [...]
Peccoud Lab Protocol: designing primers to add an N-terminal tag to the 5' end of a gene via PCR Introduction It is sometimes necessary to generate gene fusion strains with an N-terminal tag or promoter changes by introducing a PCR amplicon of a selectable marker flanked by DNA sequences of 40-60 bp homologous to the 5' [...]