Materials

Consumables:

  • Chloramphenicol (323.13 g mol -1)
  • Ethanol (96%)
  • 50mL conical vials
  • Screw cap tubes

Hardware:

  • Vortexer
  • Analytical balance

Procedure

Instructions

  1. Pipet 40mL of ethanol into a 50mL conical vial
  2. Weigh out 2g of chloramphenicol with the analytical balance
  3. Pour into ethanol and vortex
  4. Once fully dissolved, filter sterilize:
    1. Screw 0.2μm filter onto 3mL syringe
    2. Pipet antibiotic solution into syringe
    3. Plunge antibiotic solution into new 50mL conical vial
    4. Repeat until all antibiotic solution has been filter sterilized
  5. Pipet 1mL of filter sterilized antibiotic into each screw cap tube