Creation of a bacterial cell controlled by a chemically synthesized genome

Title:

Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome

Author(s):

D. G. Gibson J. I. Glass C. Lartigue V. N. Noskov R.-Y. Chuang M. A. Algire G. A. Benders M. G. Montague L. Ma M. M. Moodie C. Merryman S. Vashee R. Krishnakumar N. Assad-Garcia C. Andrews-Pfannkoch E. A. Denisova L. Young Z.-Q. Qi T. H. Segall-Shapiro C. H. Calvey P. P. Parmar C. A. Hutchison H. O. Smith J. C. Venter

Publication:

Published in: Science
Volume: 329, Issue: 5987, Pages: 52-56
Published: 07/01/2010
DOI: 10.1126/science.1190719
Website: http://www.sciencemag.org/cgi/doi/10.1126/science.1190719

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Our Discussion:

We report the design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.

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