Materials

Consumables:

  • Tris(hydroxymethyl)aminomethane (Tris) 0.6057 g
  • Disodium Ethylenedeamine tetraacetate.2H2O (EDTA) 0.1861 g
  • HCI

Hardware:

  • Mixer
  • Stir bar
  • Analytical balance
  • Graduated cylinder
  • 500 ML bottle
  • Autoclave

Prepare Buffer

  1.  Add 0.6057 g of Tris and 0.1861 g of EDTA to a 500 mL bottle with a stir bar
  2.  Add 450 ml of DI water and mix thoroughly
  3.  Add HCl until a pH of 7.6 is achieved
  4.  Add remaining DI water so a final volume of 500 mL is reached
  5. Autoclave bottle to sterilize