• Tris(hydroxymethyl)aminomethane (Tris) 181.65 g
  • DI Water
  • Concentrated HCI


  • Mixer
  • Stir bar
  • Analytical balance
  • Graduated cylinder
  • Sterile 1L bottle


  1.  Add 181.65 g of Tris to a 1L bottle with a stir bar
  2.  Add 800 ml of DI water and mix thoroughly to dissolve
  3.  Add HCI until pH of 8.8 is achieved
  4.  Add remaining DI water so a final volume of 1L is reached

Note: Make sure to let the solution cool down to room temperature before making the final pH adjustment.